Rui:DNAseq-Collaborations

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Revision as of 19:43, 17 May 2012 by >Dinh (→‎Table for samples)
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Samples

Table for samples from A.P (Belmonte lab)

hES cells dFb dFb GFP colonies P0 Notes P1 P2 P3 P5 P15 P30
gDNA gDNA A (A1, A2, A3) A1, A2 protocol test single colony single colony single colony
100c (X3) 100c (X3) B (B1, B2, B3) B2, B3 -> DNAseq single colony single colony single colony (X3) gDNA
1000c (X4) 1000c (X4) C (C1, C2, C3) single colony single colony
D (D2, D3, D4) D1 is missing single colony single colony single well single colony (X1) single well (X1)
E
F (F1, F2, F3) F1 is missing single colony single colony single colony (X1) single well (X1) single colony (X1) single well (X1)
G G is missing
HUVEC colony P0 notes P1 P2 P3 P5 P10 P15
HUVEC D (D1, D2) single colony single colony single colony (X2) single well (X2) single colony (X2) single well (X2)

Dr. Juan Carlos Izpisúa Belmonte lab

  • May 9th 2011: Athanasia iPS colonies samples in "Belmonte lab" box at -80C
  • Fibroblast samples: 2 tubes received on 11.10.11 (50k cell in 1 tube; 210k cell in 1 tube) in "collaboration" box at -80C
  • Dec 12th 2011: ES, Fibroblast samples: received on 12.12.11 in "Belmonte" box at -80C
  • March 16th 2012: all colony/clone "B", Athanasia box
P2 a single marked colony
P3 a single marked colony
P15 a single marked colony (X3)
P30 half of a dense 6-well plate (X2)

Bulk DNA isolation

  • Promega wizard genomic DNA isolation kit

Quick note

2012

<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011

<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>