Dan:Notebook/HiResChrPaint/2013-4-10

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Revision as of 19:06, 12 April 2013 by >Djacobse
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LambdaExo Digestion Gel Quantification

Urea gel to quantify Lambda Exonuclease digestion from 4/5/2013

Urea Gel Protocol

  Prerun gel for at least 20 minutes at 250V
  If the gel stops running before samples are ready just repeat
  Set up samples according to table below
Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 0.5L 1L 2L V4S1 V4S2 V4S3 V4S4 V6S1 V6S2 V6S3 V6S4 0.5L
uL Sample 0.5 1 2 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
uL ddH20 5.5 5 4 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5
uL 2x Urea Buffer 6 6 6 6 6 6 6 6 6 6 6 6
  Ran gel at 250V for 25 minutes

Results

File:LambdaExo Digest 041013.jpg File:LambdaExo Digest Sampleid 041013.jpg

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