Dan:Notebook/HiResChrPaint/2013-4-10

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Revision as of 19:14, 12 April 2013 by >Djacobse
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LambdaExo Digestion Gel Quantification

Urea gel to quantify Lambda Exonuclease digestion from 4/5/2013

Urea Gel Protocol

  Prerun gel for at least 20 minutes at 250V
  If the gel stops running before samples are ready just repeat
  Set up samples according to table below
Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 0.5L 1L 2L V4S1 V4S2 V4S3 V4S4 V6S1 V6S2 V6S3 V6S4 0.5L
uL Sample 0.5 1 2 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
uL ddH20 5.5 5 4 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5 5.5
uL 2x Urea Buffer 6 6 6 6 6 6 6 6 6 6 6 6
  Ran gel at 250V for 25 minutes

Gel Images

File:LambdaExo Digest 041013.jpg File:LambdaExo Digest Sampleid 041013.jpg

Quantification

Name Sample Volume Minus Back ng sample ng ng/uL
    INT*mm2        
U1 100 bp 0.5 uL 3734.900111 1179.300035 1.25    
U2 200 bp 0.5 uL 5710.70017 3216.350096 2.5    
U3 100 bp 1 uL 5355.74016 2704.900081 2.5    
U4 200 bp 1 uL 9250.050276 6494.800194 5    
U5 100 bp 2 uL 7312.240218 4959.430148 5    
U6 200 bp 2 uL 14064.53042 9710.270289 10    
U7 100 bp 0.5 uL 2633.210078 832.4200248 1.25    
U8 200 bp 0.5 uL 2938.250088 1623.040048 2.5    
U9 V4S1 8283.920247 2663.760079   2.60 5.19
U10 V4S2 29179.31087 15496.58046   14.15 28.29
U11 V4S3 26099.06078 14960.41045   13.66 27.33
U12 V4S4 10789.25032 5057.100151   4.75 9.50
U13 V6S2 18800.71056 12526.75037   11.47 22.95
U14 V6S3 21088.09063 13177.76039   12.06 24.12
U15 V6S4 22420.46067 15236.43045   13.91 27.82
U16   2555.600076        
U17   2494.350074        
U18   2650.840079        
U19   2755.250082        
U20   2352.81007        
U21   4354.26013        
U22   1800.790054        
U23   1315.210039        
U24   5620.160167        
U25   13682.73041        
U26   11138.65033        
U27   5732.150171        
U28   6273.960187        
U29   7910.330236        
U30   7184.030214        

Fit curve for ladder data

File:LambdaExo Digest GelQuant 041013.png


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