Daniel:Notebook/HiResChrPaint/2013-4-26
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Probe Prep Test (Started 4/22/2013)
Lambda Exonuclease Digest
1. Prepared samples according to following table
Sample | ng/uL | uL sample | uL 10x buffer | uL Lambda Exo | uL ddH20 | Total |
V4S3A | 102.2 | 30 | 5 | 2 | 13 | 50 |
V4S3B | 86.8 | 30 | 5 | 2 | 13 | 50 |
V6S3A | 80.1 | 30 | 5 | 2 | 13 | 50 |
V6S3B | 77.9 | 30 | 5 | 2 | 13 | 50 |
2. Incubate for 1 hour at 37 C 3. Heat shock 75 C 10 minutes 4. Ethanol Precipitation
Ethanol Precipitation
1. Add 250 uL nuclease free H20 to mixture (total 300 uL) 2. Add 900 uL 100% EtOH, 30 uL NaOAc, 2 uL glycoblue (2/300X) 3. Incubate overnight at -80C