Revision as of 00:06, 30 April 2013 by >Djacobse
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DpnII Digestion
Purpose: Trying to erase strange black marks from previous gels
1. Set up reactions according to table
Probe
|
ng/uL
|
uL
|
10x Buffer
|
DpnII
|
H2O
|
Total
|
V4S1
|
41.6
|
30
|
5
|
5
|
10
|
50
|
V4S2
|
159.8
|
30
|
5
|
5
|
10
|
50
|
V4S3
|
73.1
|
30
|
5
|
5
|
10
|
50
|
V4S4
|
47.8
|
30
|
5
|
5
|
10
|
50
|
V6S1
|
84.4
|
30
|
5
|
5
|
10
|
50
|
V6S2
|
111.9
|
30
|
5
|
5
|
10
|
50
|
V6S3
|
131.4
|
30
|
5
|
5
|
10
|
50
|
V6S4
|
106.9
|
30
|
5
|
5
|
10
|
50
|
2. Incubate at 37 C for 1 hr; 20 minute heat shock at 65C
3. Column purification, elute with 30 uL
4. Measure concentration in Nanodrop
Nanodrop Results
Probe
|
ng/uL
|
uL
|
ng
|
V4S1
|
21.9
|
30
|
657
|
V4S2
|
120.6
|
30
|
3618
|
V4S3
|
53.6
|
30
|
1608
|
V4S4
|
25
|
30
|
750
|
V6S1
|
61.2
|
30
|
1836
|
V6S2
|
82
|
30
|
2460
|
V6S3
|
105.3
|
30
|
3159
|
V6S4
|
80.6
|
30
|
2418
|