Hosuk:LabNotes/2013-5-2
Jump to navigation
Jump to search
Reverse Transcription check
- based on Debugging plan (05/01)
Result
- RT process seemed work.
- +RTase sample showed very strong Cy5 signal which is supposed cDNA
- comparing to -RTase result, the bright singal over the cell seemed they were cDNAs
- -RTase sample has very low intensity of signals in the cell, and
- not many spots were observed, which spots were outside of cells.
- Most of signal came from cell structure.
- The picture of -RTase is the position where the most number of spots were observed in all over the area.
- In the +RTase sample, similar number or density of spots were observed in all over the area.
- I don't know what are the large spots. Are they also cDNAs?
- +RTase
S2:+RTase, Brighfield, 20x | S2:+RTase, Cy5, 20x | S2:+RTase, Merged | |||
(Original) | (Original) | ||||
File:RTCheck +RTase fig08 20xobj pos04 BF exp0.2-EM00.jpg | File:RTCheck +RTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg | File:Composite +RTase fig07-08.jpg |
- -RTase (No RTase)
S3:-RTase, Brighfield, 20x | S3:-RTase, Cy5, 20x | S3:-RTase, Merged | |||
(Original) | (Original) | ||||
File:RTCheckNoRTase fig08 20xobj pos05 BF exp0.2-EM00.jpg | File:RTCheckNoRTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg | File:Composite NoRTase fig07-08.jpg |
CircLigase II reaction on the dish
- based on Debugging plan (05/01)