Kun:LabNotes/ASE/2007-10-29
Jump to navigation
Jump to search
Exp1: Probe prep (cont.)
- Starting from ethanol precipitated DNAs made at 10/18/2007.
- Pool two tubes of the same amplicons into ~100ul in total, add 11ul 10x Lambda Exo buffer, 4ul 5U/ul Lambda exonuclease. Incubate @ 37C for 2 hours, check 2ul of the products in both 8% TBE gels and 6% TBU gels.
8% TBE gel 6% TBU gel File:2007-10-29-PCR-Lambda exo-TBE.jpg File:2007-10-29-PCR-Lambda exo-TBU.jpg
- I saw sharp bands in the non-denaturing gel, suggesting there are still a lot of dsDNA. I decided to incubate the reactions for another 4 hours.