Matt:LabNotes/2013-5-17

From ZhangLabWiki
Revision as of 20:51, 17 May 2013 by >Mzcai
Jump to navigation Jump to search

Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16

PAGE-Size Selection of 20 gap probes

  • Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield
  • I will run 3 gels each with 60ul (~3.4 ug of DNA each)

Sample

Components 1X Volume 3X Volume
Enzyme digested probes (~10ug/180ul) 60 180
TBE-Urea Buffer 2X 60 180
Water 0 0
Total 120 360

3 Low Mass DNA Ladders

Components 3X Volume
Low Mass DNA ladder (1 ug/ul) 3
TBE-Urea Buffer 2X 15
H2O 12
Total 30