Hosuk:LabNotesMDA/2013-6-21
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MDA with PGP1F, 4th - qPCR
- MDA run @06/19
- qPCR result was not correct because of using wrong primers --> re-run
- For the initial test, 7 samples - 2, 5, 11, 13, 18, 20, 22 were run.
10ng | 10ng | 1ng | 1ng | 100pg | 100pg | 10pg | 10pg | |||||||
NTC | #2 | #5 | #11 | #13 | #18 | #20 | #22 | |||||||
Result --> Fail!
- Ladders were correct, but samples were not shown any PCR amplification.
- MDA didn't work.
- Possible reason would be
- Experimental error, or
- Re-hybridization while Neutralization?
- Run one more time with the same condition but keeping 1 min. incubation carefully while neutralization, (not too long)