Hosuk:LabNotesMDA/2013-6-21

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Revision as of 18:30, 21 June 2013 by >Hosuki78
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MDA with PGP1F, 4th - qPCR

  • MDA run @06/19
  • qPCR result was not correct because of using wrong primers --> re-run
    • For the initial test, 7 samples - 2, 5, 11, 13, 18, 20, 22 were run.
10ng 10ng 1ng 1ng 100pg 100pg 10pg 10pg
NTC #2 #5 #11 #13 #18 #20 #22


Result --> Fail!

  • Ladders were correct, but samples were not shown any PCR amplification.
  • MDA didn't work.
  • Possible reason would be
    • Experimental error, or
    • Re-hybridization while Neutralization?
  • Run one more time with the same condition but keeping 1 min. incubation carefully while neutralization, (not too long)