Hosuk:LabNotesMDA/2013-7-22

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MDA with PGP1F, 9th, Dev v9.1

  • Use new device (v9.1)
  • Same experiment to 07/18, but try to run MDA in ALS chamber after transferring solution into compartments.


Experiment

  • NS valve didn’t work properly but the reaction was kept going. (8hr reaction)


Result

  • Not bad, considering bad experiment condition
  • Ch25 is the reaction in ALS chamber.
    • The amount of amplicon was estimated 483.26pg/uL, which is 724.89pg in total.
    • While transferring ALS+Cell solution into compartment with air, the remained solution was kept drying (evaporation by air)
    • Thus using H2O would be better than air.