Daniel:Notebook/GenomeMiner/2013-8-19

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Revision as of 18:53, 27 August 2013 by >Djacobse
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HL155

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Redoing analysis using Phred 64

Creating build index

 bowtie2-build -f Probelist_all.fa hrcp_probesequences &

Running Bowtie 2 with Phred 64 flag

bowtie2 -x hrcp_probesequences -U  
s_2_1_Indx12.txt,s_2_1_Indx53.txt,s_2_1_Indx54.txt,s_2_1_Indx04.txt,s_2_1_Indx03.txt,s_2_1_Indx05.txt,s_2_1_Indx02.txt,
s_2_1_Indx13.txt,s_2_1_Indx01.txt,s_2_1_Indx55.txt,s_2_1_Indx49.txt,s_3_1_Indx11.txt,s_3_1_Indx10.txt,s_2_1_Indx56.txt,
s_2_1_Indx52.txt,s_3_1_Indx12.txt,s_2_1_Indx11.txt,s_2_1_Indx50.txt --phred64 -S hrcp_fullindex_samout_p64.out