Hosuk:LabNotesMDA/2013-8-27
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MDA with PGP1F, 14th, Dev v9.1
- Use mineral oil in valve lines, Water in valves for cell capture and mineral oil for rest of valves
- 10hr reaction
Result
- Result : Not bad…but
- Pushing water doesn’t look good. Chromosome wasn’t pushed completely.
- Ch 1, 2, 3 had not enough template, and MDA product.
- Pushing air and run MDA in denature chamber…