Daniel:Notebook/HiResChrPaint/2013-9-4

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BSPP Control (Started 09/03/2013)

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DpnII Digestion (Started 09/03/2013)

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Nanodrop Results

TBE Gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 25bp ladder BSPP 1CD BSPP 2AB BSPP 2CD
2. Run for 25 minutes at 200V

Lambda Exonuclease Digestion