Blue:RNA-Seq Experiments:09052013

From ZhangLabWiki
Revision as of 23:37, 10 September 2013 by >B1lake (→‎Fluidigm C1 Small Chip Beta Test)
Jump to navigation Jump to search

Fluidigm C1 Small Chip Beta Test

  • Tested NPCs on the small 5-10um chip
  • First run on 09-04-2013 produced too few viable cells for loading and resulted in only 10 captured - therefore, reprimed the chip and stored overnight for re-loading
  • Second run on 09-05-2013 involved E13.5 neural progenitors sorted as PI negative
  • Achieved good loading efficiency (81/96) but high mortality as indicated by live/dead staining on chip
  • Followed the protocol exactly - including use of spike-in RNA indicated in the protocol rather than those used in our previous experiments


File:Cell Characterization Worksheet.docx

File:Data Submission Worksheet.docx