Daniel:Notebook/HiResChrPaint/2013-9-30

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Revision as of 19:05, 3 October 2013 by >Djacobse (→‎Fibroblast Culture)
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Fibroblast Culture

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Split passage 9 cells into 3 culture dishes, also prepped 5 plates for fixing/hybridization.

Prepping cells for hybridization

1. Added 100 uL of cell suspension (after step 5) to center of petri dish
2. Incubated for ~9 hours
3. Aspirated media and added 1 mL fresh DMEM
4. Incubated overnight

FISH (Started 9/27/2013)

Microscopy

Performed microscopy on samples from 9/27 using Olympus microscope upstairs.

Results

Control

Dan_exp_overlay_bfdapigfp_40x_FISH_20130930.png
40x magnification; BF,DAPI,GFP