Daniel:Notebook/HiResChrPaint/2013-9-30
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Fibroblast Culture
Split passage 9 cells into 3 culture dishes, also prepped 5 plates for fixing/hybridization.
Prepping cells for hybridization
1. Added 100 uL of cell suspension (after step 5) to center of petri dish 2. Incubated for ~9 hours 3. Aspirated media and added 1 mL fresh DMEM 4. Incubated overnight
FISH (Started 9/27/2013)
Microscopy
Performed microscopy on samples from 9/27 using Olympus microscope upstairs.
Results
Control
File:Dan exp overlay bfdapigfp 40x FISH 20130930.png |
40x magnification; BF,DAPI,GFP |
File:Dan exp overlay bfdapitxred 10x FISH 20130930.png | File:Dan exp overlay bfdapitxred 20x FISH 20130930.png |
10x magnification; BF,DAPI,TXRED | 20x magnification; BF,DAPI,TXRED |
File:Dan exp overlay bfdapitxred 40xFISH 20130930.png | File:Overlay-all FISH 20130930.png |
40x magnification; BF,DAPI,TXRED | 40x magnification; BF,DAPI,TXRED,GFP |