Daniel:Notebook/HiResChrPaint/2013-9-30

From ZhangLabWiki
Revision as of 19:08, 3 October 2013 by >Djacobse (→‎Results)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Fibroblast Culture

Back to Calendar

Split passage 9 cells into 3 culture dishes, also prepped 5 plates for fixing/hybridization.

Prepping cells for hybridization

1. Added 100 uL of cell suspension (after step 5) to center of petri dish
2. Incubated for ~9 hours
3. Aspirated media and added 1 mL fresh DMEM
4. Incubated overnight

FISH (Started 9/27/2013)

Microscopy

Performed microscopy on samples from 9/27 using Olympus microscope upstairs.

Results

Control

File:Dan exp overlay bfdapigfp 40x FISH 20130930.png
40x magnification; BF,DAPI,GFP


File:Dan exp overlay bfdapitxred 10x FISH 20130930.png File:Dan exp overlay bfdapitxred 20x FISH 20130930.png
10x magnification; BF,DAPI,TXRED 20x magnification; BF,DAPI,TXRED
File:Dan exp overlay bfdapitxred 40xFISH 20130930.png File:Overlay-all FISH 20130930.png
40x magnification; BF,DAPI,TXRED 40x magnification; BF,DAPI,TXRED,GFP