Daniel:Notebook/HiResChrPaint/2013-10-3

From ZhangLabWiki
Revision as of 18:49, 3 October 2013 by >Djacobse (→‎Results)
Jump to navigation Jump to search

FISH (Started 10/01/2013)

Back to Calendar

Microscopy

Used the Olympus Microscope upstairs to view the most recent round of FISH slides.

Results

Control (BSPP-2)

File:Overlay con 10x FISH2013 10 03.png File:Overlay con 40x FISH2013 10 03.png
10x magnification; BF,DAPI,TXRED 40x magnification; BF,DAPI,TXRED

Experiment (V6S1+V6S3)

File:Overlay exp 20x FISH 2013 10 03.png
20x magnification; BF,DAPI,TXRED

Discussion

The control sample worked well enough, it seems like there is plenty of signal but it isn't in any particular place. The experiment group, however, looked terrible, as it looked like there were no cells anywhere on the dish. Further, there were scratches everywhere, distorting the view. I'll have to make sure on future samples that the cells, after fixation, look healthy and plentiful.