Daniel:Notebook/HiResChrPaint/2013-10-23

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Revision as of 18:41, 23 October 2013 by >Djacobse (→‎BSPP Control (Started 10/22/2013))
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BSPP Control (Started 10/22/2013)

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DpnII Digestion (Started 10/22/2013

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Nanodrop Results

Sample BSPP 1AB BSPP 1CD BSPP 2AB BSPP 2CD
ng/uL 166.5 168.8 180.2 143
ug in 30 uL 5.0 5.1 5.4 4.3
total   10.1   9.7

TBE Gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7.2 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 10bp ladder BSPP 1AB BSPP 1CD BSPP 2AB BSPP 2CD
2. Run for 23 minutes at 220V
3. Stain with 3 uL SYBR gold for 2 minutes

Gel Image

Lambda Exonuclease Digestion

1. Reaction table


2. Incubate for 1 hour at 37C followed by 10 minute heat inactivation at 75C
3. Pool samples
4. ssDNA column purification, elute with 20 uL nfH2O
5. Measure Concentration in Nanodrop

ssNanodrop Results