AlanFung:LabNotes/Capturing/2014-1-23
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Capturing ppMALAT1 template w ppMALT1 ordered form IDT
TBU GEL to verify probe template size
- Aim for 100ng/band
- 10bp Ladder
- ppMALAT1 (92nt, 100uM=3036ng/ul)
- MALAT1 Template (45nt, 100uM=1485ng/ul)
- ppillumina_155 (99nt, 100uM=3267ng/ul)
Sample | ng/ul | ul | ng/ul | ul | H2O |
ppMALAT1 | 3036 | 0.99 | 100 | 30 | 29.01 |
MALAT1 Template | 1485 | 1.35 | 100 | 20 | 18.65 |
ppillumina_155 | 3267 | 0.92 | 100 | 30 | 29.08 |
10BP | 1000 | 0.10 | 100 | 1 | 99 |