Hosuk:LabNotes/2014-2-3
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NCIH1975 : Mutation Detection : 2nd Try
- Use dcProbe1~4 FAM and Cy3 to decode Wild Type and Mutation of EGFR T790M and L858R
- Step1 : dcProbe1-FAM
- Step2 : dcProbe1-Cy3
- Step3 : dcProbe2-FAM
- Step4 : dcProbe2-Cy3
- Step5 : dcProbe3-FAM
- Step6 : dcProbe3-Cy3
- Step7 : dcProbe4-FAM
- Step8 : dcProbe4-Cy3
- Further analysis is on going.