EricChu:LabNotesMDA/2014-2-5

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qPCR Alu and Mito of MDA generated libraries on 1-30-2014

  • These libraries were prepared by tagmentation.
  • 1ul of each library was used in a 20ul qPCR reaction.
  • 1ul 10ng human gDNA was used as control.

File:02052014qPCR.jpg

  • Alu primers were labeled black and Mito primers were labeled red.
  • Alu curves of both libraries on 1-30-2014 (labeled Hi and Lo) were amplified later than their corresponding Mito curves. However, as seen in gDNA, Alu should be amplified earlier than Mito.
  • In this experiment, most of the DNA were coming from mitochondrial DNA rather than chromosomal DNA.