Daniel:Notebook/HiResChrPaint/2014-2-16
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Multi-Color FISH (Started 02/08/2014)
Cot1 Labeling (Started 2/14/2014)
- Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
- Remove supernatant and replace with 750uL 75% EtOH
- Centrifuge for 10 minutes at 12000 RPM at 4 C
- Remove supernatant and leave in hood to dry
- Resuspend each sample in 20 uL nfH2O
- Leave overnight, continued tomorrow