Song Chen:Daily/NOTES/2014-2-26
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Naive condition cultured H9 ES cells
Setting: Expose 1000 ms, PFBH 406, 10x obj,
NANOG/SOX2
File:022614 NHSM NANOG.png | File:022614 NHSM SOX2.png | File:022614 NHSM DAPI.png |
OCT4/SOX2
File:022614 NHSM OCT4.png | File:022614 NHSM SOX2.2.png | File:022614 NHSM DAPI.2.png |
H9 hESCs cultured in naive condition express OCT4,SOX2 and NANOG. While SOX2 and NANOG expression are very clear, OCT4 expression is still dim, and sometimes there are some OCT4 high cells with flatten morphology.
SSEA4 is still unclear, YAP shows majority nuclear localization in hES colonies.