Matt:LabNotes/2014-6-19
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Check Agi26k0gap Oligos for DpnII Restriction Sites
- Lambda exonuclease digestion has been having low efficiency Try 1 and Try 2
- Dr. Zhang suggested skipping lambda if very few oligos would be digested
- Used ProbesWithDpnIISites.pl to identify oligos with extra DpnII site
- Oligos identified by line # in the text output
- Of 12,964 oligos, 8,744 (67%) have DpnII restriction sites besides the intended cleavage site
- Conclusion: cannot skip lambda digestion step