Daniel:Notebook/HiResChrPaint/2014-7-18
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Dystrophin Probes
The dystrophin probes I designed last week arrived from IDT. Attached are their specs:
The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:
Oligo Pool Synthesis
- Resuspend each oligo to make them all 1 mM (See Plate Specs for values)
- Combine 2 uL from each oligo, makes 12.5 ug/uL total pool
- Total volume will be 64 uL
- Add 742.4 uL nfH2O, makes 1 ug/uL total pool