Matt:LabNotes/2014-8-4

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Revision as of 21:39, 5 August 2014 by >Mzcai (→‎Column Purification)
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Agi26k0gap Probe Production Continued

TBU Gel Check

  • 2ul Sample + 2ul 2X Urea Loading Dye
  • 0.5ul Low Mass Ladder + 1.5ul H2O + 2ul 2X Urea Loading Dye
  • Digested sample
  • Undigested sample: 10nM 1st round amplicon

File:2014-08-04 Agi26k0gapDigestCheck.jpg

  • Brightest band below 200bp as expected

Column Purification

  • Used Zymo ssDNA/RNA columns
  • Total Eluted: 124 ul
  • Nanodrop quant: 137.6 ng/ul
  • Yield: 137.6 ng/ul x 124 ul = 17.06 ug -->