Matt:LabNotes/2014-8-4
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Agi26k0gap Probe Production Continued
- Continued from: Matt:LabNotes/2014-7-22
- Thaw and combine the 7 tubes of 200ul that were stored at -20C
TBU Gel Check
- 2ul Sample + 2ul 2X Urea Loading Dye
- 0.5ul Low Mass Ladder + 1.5ul H2O + 2ul 2X Urea Loading Dye
- Digested sample
- Undigested sample: 10nM 1st round amplicon
File:2014-08-04 Agi26k0gapDigestCheck.jpg
- Brightest band below 200bp as expected
Column Purification
- Used Zymo ssDNA/RNA columns
- Total Eluted: 124 ul
- Nanodrop quant: 137.6 ng/ul
- Yield: 137.6 ng/ul x 124 ul = 17.06 ug -->