Hosuk:LabNotes/2014-8-10

From ZhangLabWiki
Revision as of 23:11, 12 August 2014 by >Hosuki78
Jump to navigation Jump to search

Cancer Mutation detection

Decoding, and Analyzing

  • Images
    • ch00 - dcProbe[#]-488
    • ch01 - dcProbe[#]-Cy3
    • ch02 - Bright field
  • Steps
    • Step1 : dcProbe0-488
    • Step2 : dcProbe0-Cy3
    • Step3 : dcProbe1-488
    • Step4 : dcProbe1-Cy3
    • ...
    • Step9 : dcProbe4-488
    • Step10 : dcProbe4-Cy3


  • I've taken images from 3 different positions (Pos1, Pos2, and Pos3)
    • However the result that successfully analyzed was from only Pos1


  • Procedure
    1. Figure out the offset pixels with BF image
    • Pos1
    • Standard : Step3
    • Step1 : X = 1, Y = -25
    • Step2 : X = 0, Y = -8
    • Step3 : X = 0, Y = 0
    • Step4 : X = -8, Y = 2
    • Step5 : X = -8, Y = 9


    1. Make maximum intensity projection(MIP) images at each step