Hosuk:LabNotes/2014-8-10
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Cancer Mutation detection
Decoding, and Analyzing
- Images
- ch00 - dcProbe[#]-488
- ch01 - dcProbe[#]-Cy3
- ch02 - Bright field
- Steps
- Step1 : dcProbe0-488
- Step2 : dcProbe0-Cy3
- Step3 : dcProbe1-488
- Step4 : dcProbe1-Cy3
- ...
- Step9 : dcProbe4-488
- Step10 : dcProbe4-Cy3
- I've taken images from 3 different positions (Pos1, Pos2, and Pos3)
- However the result that successfully analyzed was from only Pos1
- Procedure
- Figure out the offset pixels with BF image
- Pos1
- Standard : Step3
- Step1 : X = 1, Y = -25
- Step2 : X = 0, Y = -8
- Step3 : X = 0, Y = 0
- Step4 : X = -8, Y = 2
- Step5 : X = -8, Y = 9
- Make maximum intensity projection(MIP) images at each step