Hosuk:LabNotes/2014-8-19
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Decoding with Agi26k0gap Padlock Probe
- Human brain tissue from Yun
- HB3, section 9
- Total 15 tissue sections
- Mouse tissue : Total 15 tissue sections
- Stored in -80C
Procedure
Fixation
- 4% Formaldehyde : 2.5mL 16% Paraformaldehyde + 1mL 10x DEPC-PBS + 6.5mL DEPC-H2O (Tot. 10mL)
- 16% Paraformaldehyde Solution)
- Manufacture : Electron Microscopy Diatome --> 16% PARAFORMALDEHYDE AQ SOLUTN 50-980-486, No.:15700
- Supply : (Fisher Scientific), 50980486
- Paraformaldehyde - methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. Easy to break, prescored, 10 ml ampoules sealed under inert gas 10 ml
- Take Tissue glass slide from -80C, and put them to room temp. for 2min.
- Add 4% formamide on a tissue, incubate for 30min at room temp.
- Wash with PBS 3 times (add PBS in 3 wells of 6 well plate and series washing with glasses)
- Attach glass with glue
- Cyanoacrylate adhesives : High-viscosity, Sigma-Aldrich, Z105902-1EA
- Wipe PBS from the glass (around tissue)
- Apply glue along the edge of the glass, put on the bottom of MatTek dish, wait for 5min
File:TissueGlassOnMatTek 2.png File:TissueGlassOnMatTek 1.png