AlanFung:LabNotes/2014/2014-9-8

From ZhangLabWiki
Jump to navigation Jump to search

qPCR Test to verifiy End repair, A-tailing & Adapter Ligation

Component 1 Library 19 Libraries + 3%
qPCR Master Mix:    
Adapter ligated DNA 1 19.6
10uM PCR_F 0.5 9.8
10uM PCR_R.Ind2 0.5 9.8
2x iQ MM 12.5 244.6
Water 10.5 205.5
Total master mix volume 25 489.3
  • 98C 2min --> (98C 15sec -> 64C 20sec -> 72C 30sec) x 12 cycles --> 72C 3min --> 15C Hold

PAGE Gel Verification