Noi/NOTES/2014-8-18

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Revision as of 19:27, 13 September 2014 by >Noi
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Preparation of WGBS libraries of small fragment DNA in cancer patient plasma samples (no DNA fragmentation step, REPEAT with dAdGdCdT for end repair)

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  • There is one sample change for sample in colon cancer in 2014-08-03 note. Sample 6-P-6 was replace with sample 6-P-10.

1) End-repair/dA-tailing

  • Incubate plasma DNA with 5U Klenow fragment, exo- and dA:dC:dG:dT (final conc. 1:0.1:0.1:0.1mM) in total volume 20ul