Daniel:Notebook/HiResChrPaint/2014-9-23
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Slide FISH (Started 09/19/2014)
Microscopy
For today's microscopy, I tried something new in order to help determine some of the properties of the microscope/sample. I took an image series for both the DMD and the PPRL samples using different resolutions in the following order (values are for one side, total image is number of pixels squared): 2048 pixels, 2048 pixels, 1024 pixels, 512 pixels, 256 pixels, 2048 pixels. All other properties, gain, x,y,z locations, etc. were kept constant. This will allow me to see (a) if the resolution of the image seems to matter and (b) fluorophore quenching from multiple rounds of imaging.
DMD-488 and Cot1-546
- 20140923 DMD488 Cot1546.lif Series 2048pxSnapshot.jpg
2048 pixels (1)
- 20140923 DMD488 Cot1546.lif Series 2048px 2Snapshot.jpg
2048 pixels (2)
- 20140923 DMD488 Cot1546.lif Series 1024px 3Snapshot.jpg
1024 pixels (3)
- 20140923 DMD488 Cot1546.lif Series 512x 4Snapshot.jpg
512 pixels (4)
- 20140923 DMD488 Cot1546.lif Series 256px 5Snapshot.jpg
256 pixels (5)
- 20140923 DMD488 Cot1546.lif Series 2048px 6Snapshot.jpg
2048 pixels (6)