Noi:Labnote tracking
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Labnote tracking
Date Description December 2014 2014-12-11 RRBS library prep, mouse hippocampus gDNA (Zhou's lab) 2014-12-08 LMS + CRC BSPP capture on WGBS libraries prepared from ctDNA of CRC patient (Illumina) 2014-12-05 WGBS library prep and BSPP capture of cfDNA from Illumina (with Li Liu) ctDNA of colorectal cancer (CRC) patients from Illumina (samples from Kansas University) 2014-12-04 SeqCap Epi probe pool (UMR v1) hybridization capture on cancer patient WGBS libraries (plasma/tumor tissue DNA, ) 2nd experiment (used 3 tubes of LMS SeqCap probe pool)
November 2014 2014-11-25 Comparison of the efficiency between TruSeq adaptor and a customized unmethylated Y-adaptor with UMI 14-12-04 SeqCap Epi probe pool (UMR v1) hybridization capture on normal and cancer patient WGBS libraries 1st Experiment (used 2 tubes of LMS SeqCap probe pool) Also included Part I: Re-amplification of WGBS libraries of normal plasma samples
October 2014 2014-10-31 Repeating of BIS/DNA SMART ChIP-Seq protocol 2014-10-29 Generation of sequencing library of a customized SeqCap EZ Epi Probe Pool to decode the sequences of LMS SeqCap probe pool Library generated by DNA SMART ChIP Seq Kit 2014-10-24 Preparation of WGBS of cfDNA from plasma using KAPA Hyper Prep kit & NEXTflex Cell Free DNA-Seq kit To compare the efficiency between the two commercial kits Repeated experiment of 14-1022 2014-10-22 Preparation of WGBS of cfDNA from plasma using KAPA Hyper Prep kit 2014-10-15 Expansion PCR of new probe set from CustomArrays (90k_oligos_30Sept2014) Bad oligos. Got re-synthesized of the new oligos by CustomArrays The new tube was amplified by Rui on 14-11-12 2014-10-08 Integration of DNA SMART ChIP-Seq protocol with RRBS for low input DNA methylation assay