Noi:Labnote tracking

From ZhangLabWiki
Revision as of 12:41, 24 December 2014 by >Noi
Jump to navigation Jump to search

Labnote tracking

Back

Date                         Description
December 2014
2014-12-11                   RRBS library prep, mouse hippocampus gDNA (Zhou's lab)
2014-12-08                   LMS + CRC BSPP capture on WGBS libraries prepared from ctDNA
                             of CRC patient (Illumina)
2014-12-05                   WGBS library prep and BSPP capture of cfDNA from Illumina (with Li Liu)
                             ctDNA of colorectal cancer (CRC) patients from Illumina 
                             (samples from Kansas University)
2014-12-04                   SeqCap Epi probe pool (UMR v1) hybridization capture on cancer patient
                             WGBS libraries (plasma/tumor tissue DNA, )
                             2nd experiment (used 3 tubes of LMS SeqCap probe pool)
November 2014
2014-11-25                   Comparison of the efficiency between TruSeq adaptor and a customized 
                             unmethylated Y-adaptor with UMI
14-12-04                     SeqCap Epi probe pool (UMR v1) hybridization capture 
                             on normal and cancer patient WGBS libraries 
                             1st Experiment (used 2 tubes of LMS SeqCap probe pool)
                             Also included 
                             Part I: Re-amplification of WGBS libraries of normal plasma samples
October 2014
2014-10-31                   Repeating of BIS/DNA SMART ChIP-Seq protocol
2014-10-29                   Generation of sequencing library of a customized SeqCap EZ Epi Probe Pool 
                             to decode the sequences of LMS SeqCap probe pool
                             Library generated by DNA SMART ChIP Seq Kit
2014-10-24                   Preparation of WGBS of cfDNA from plasma using KAPA Hyper Prep kit & 
                             NEXTflex Cell Free DNA-Seq kit
                             To compare the efficiency between the two commercial kits
                             Repeated experiment of 14-1022
2014-10-22                   Preparation of WGBS of cfDNA from plasma using KAPA Hyper Prep kit
2014-10-15                   Expansion PCR of new probe set from CustomArrays (90k_oligos_30Sept2014)
                             Bad oligos. Got re-synthesized of the new oligos by CustomArrays 
                             The new tube was amplified by  Rui on 14-11-12
2014-10-08                   Integration of DNA SMART ChIP-Seq protocol with RRBS for low input DNA methylation assay