Daniel:Notebook/RNAFISH/2015-1-29
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Oligo Set Preparations
These are the oligos that I ordered on 08/04/2014. Now that I am ready to do the experiment, I need to prep them. First thing is to resuspend the oligos. By order, each oligo has 15 nmol.
- Resuspend each oligo in 15 uL nfH2O (1 mM)
- Take 2 uL from each oligo well and combine them in a single 1.5 mL tube (total concentration 1 mM)
- Store the remaining oligos at -20C
- Add 384 uL nfH2O, diluting 5:1, for final concentration of 200 uM
- Check concentration in nanodrop at 1:1, 5:1, and 10:1 dilutions for accuracy
Nanodrop Results
1:1 ng/uL | 5:1 ng/uL | 10:1 ng/uL | Average (ng/uL) | STDev (ng/uL) | |
FOXP2 | 959.3 | 194.2 | 97.5 | 968.4 | 8.2 |
GAD1 | 951.9 | 190 | 94.7 | 949.6 | 2.5 |
So they are both just under 1 ug/uL.