Revision as of 22:30, 14 July 2015 by >Mzcai
Decoding MotorNeurons_DARTFISHsuppv2
Procedure
- Did universal Cy3 adpt + 5 cycle decoding on 8 random positions in the dish (Cy5, Cy3, 488)
- 63x objective, 1x zoom = 184um x 184um FOV
- 2048pixels x 2048pixels
- 0.49um z-step size, ~14um total imaging depth
Results
- Saved in: Documents\RolonyImages\7-11-2015\MotorNeurons_DARTFISHsuppv2\Aligned\MotorNeurons_07-11-2015
- Summary results for 8 positions
- Dropbox\GradZhangLab\DecodeData
Pos6 Example
File:Comp MotorNeurons 07-11-2015 Pos6 Try 1.png
Color
|
Gene
|
Count
|
Red |
MALAT1 |
146
|
Lime |
NEFL |
58
|
Blue |
KIF5C |
43
|
Yellow |
SLIT2 |
34
|
Cyan |
UBE3A |
26
|
Magenta |
MEIS2 |
24
|
Silver |
MIAT |
19
|
Maroon |
SLIT3 |
16
|
Olive |
ZFHX3 |
16
|
Green |
TENM3 |
15
|
Purple |
SNAP25 |
14
|
Teal |
SEMA6D |
13
|
Navy |
SEMA3C |
11
|
Maroon |
QKI |
10
|
Dark red |
THSD7A |
10
|
Top 20 Detected Genes
Gene
|
Count
|
MALAT1 |
1167
|
NEFL |
516
|
KIF5C |
383
|
UBE3A |
224
|
SLIT2 |
219
|
MEIS2 |
184
|
MIAT |
145
|
SEMA6D |
145
|
ZFHX3 |
138
|
THSD7A |
109
|
QKI |
100
|
TENM3 |
96
|
SEMA3C |
89
|
SLIT3 |
84
|
PHKB |
78
|
SNAP25 |
76
|
PPFIBP1 |
71
|
PCDH7 |
69
|
CTNNA2 |
66
|
DACH1 |
56
|
Top 25 Normalized Detected Genes
- Normalized with CA12kNov14suppv2-gDNA_H1H2_sorted_filtered_Genecounts.txt
Gene
|
Count
|
invitro
|
Normalized
|
MALAT1 |
1167 |
442 |
2.640
|
NEFL |
516 |
418 |
1.234
|
MEIS2 |
184 |
294 |
0.626
|
TOM1 |
13 |
25 |
0.520
|
VAV2 |
4 |
11 |
0.364
|
NAPA |
4 |
12 |
0.333
|
SEMA5B |
45 |
143 |
0.315
|
DLGAP4 |
21 |
67 |
0.313
|
KIF5C |
383 |
1248 |
0.307
|
SLIT2 |
219 |
812 |
0.270
|
SLIT3 |
84 |
339 |
0.248
|
OLFM1 |
14 |
57 |
0.246
|
NRG1 |
14 |
58 |
0.241
|
TIMP2 |
44 |
191 |
0.230
|
SHISA7 |
14 |
62 |
0.226
|
GPR56 |
42 |
245 |
0.171
|
QKI |
100 |
688 |
0.145
|
MIAT |
145 |
1048 |
0.138
|
FAIM2 |
13 |
94 |
0.138
|
NPTX1 |
3 |
22 |
0.136
|
CUX2 |
26 |
191 |
0.136
|
ZFHX3 |
138 |
1073 |
0.129
|
LRP1 |
11 |
95 |
0.116
|
SEMA6D |
145 |
1517 |
0.096
|
GRIP2 |
2 |
23 |
0.087
|