Sam:LabNotes/Microbiome/2009-3-9 exp2

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Revision as of 04:13, 10 March 2009 by >Sam Chiang
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Multiple PCR against human whole genomoe

Objective

  • Based on the previous multiple PCR result, increase the primer concentration for those weak bands
  • Test the effect of Q-solution come with Qiagen multiple PCR kit


Material and Samples

  • Prepare the new primer gourp mix for primer set-1(1X = 10 uL, 2X = 20 uL)
    • Set-1 Blue: including Y-1(2X), 21-1(2X), the rest primers (1X)
    • Set-1 Yellow: 11-1(2X), 10-1(2X), 16-1(2X)
    • Set-1 Red: Use the old one
    • Set-1 Green: 18-1(2X), 3-1(2X)
    • Set-1 Orange: 20-1(2X), 7-1(2X)

Procedures