Brandon:LabNotes/Project1/2015-9-18

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combinatorial method sequences detail

i5 universal connector
AATGATACGGCGACCACCGAGATCTACAC  i5_nXTv2 index primer
AATGATACGGCGACCACCGAGATCTACAC[CTCTCTAT]TCGTCGGCAGCGTC i5 barcode + flowcell adaptor
AATGATACGGCGACCACCGAGATCTACAC[TATCCTCT]TCGTCGGCAGCGTC 
AATGATACGGCGACCACCGAGATCTACAC[GTAAGGAG]TCGTCGGCAGCGTC                 
                                                                         GCGATCGAGGACGGC [AGATGTGTATAAGAGACAG]  read1
                                       TCGTCGGCAGCGTC{TCCACGC}[TATAGCCT]{GCGATCGAGGACGGC}[AGATGTGTATAAGAGACAG]  i5_transposon + bc
                                       TCGTCGGCAGCGTC{TCCACGC}[ATAGAGGC]{GCGATCGAGGACGGC}[AGATGTGTATAAGAGACAG]  
                                                                      

i7 universal connector
                                        CACCGTCTCCGCCTC [AGATGTGTATAAGAGACAG] read 2
GTCTCGTGGGCTCGG{CTGTCCCTGTCC}[CGAGTAAT]{CACCGTCTCCGCCTC}[AGATGTGTATAAGAGACAG]
GTCTCGTGGGCTCGG{CTGTCCCTGTCC}[TCTCCGGA]{CACCGTCTCCGCCTC}[AGATGTGTATAAGAGACAG]

                                   
                                                            GGCTCGGGTGCTCTG[ATTCCGCT]TAGAGCATACGGCAGAAGACGAAC i7 bc+ flowcell adaptor
                                                            GGCTCGGGTGCTCTG[GCTCCGAC]TAGAGCATACGGCAGAAGACGAAC
GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC} <- read2
GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC}[TAATGAGC]{CCTGTCCCTGTC}GGCTCGGGTGCTCTG i7_transposon = bc
GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC}[AGGCCTCT]{CCTGTCCCTGTC}GGCTCGGGTGCTCTG
CTGTCTCTTATACACATCT{GAGGCGGAGACGGTG} -> index 1