Daniel:Notebook/RNAFACS/2015-12-1
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PA Gel Slide Testing
Didn't have time today to start testing, so I'll do it tomorrow. Today I prepped fresh ALS and NS and tested against each other. From now on, I'll use the below as my formula.
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Buffers
- Alkaline Lysis Solution (ALS)
- 80 ul Nuclease free H2O.
- 8 ul 5 M KOH.
- 10 ul 1 M DTT.
- 2 ul 0.5 M EDTA
- Neutralization Solution (NS)
- 65 uL H20
- 15 uL 4M Tris-HCl
- 20 uL 2N HCl