Daniel:Notebook/RNAFACS/2015-12-1

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Revision as of 16:53, 2 December 2015 by >Djacobse (→‎PA Gel Slide Testing)
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PA Gel Slide Testing

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Didn't have time today to start testing, so I'll do it tomorrow. Today I prepped fresh ALS and NS and tested against each other. From now on, I'll use the below as my formula. |

Buffers

  • Alkaline Lysis Solution (ALS)
    • 80 ul Nuclease free H2O.
    • 8 ul 5 M KOH.
    • 10 ul 1 M DTT.
    • 2 ul 0.5 M EDTA
  • Neutralization Solution (NS)
    • 65 uL H20
    • 15 uL 4M Tris-HCl
    • 20 uL 2N HCl