Daniel:Notebook/ComboLock/2016-3-11
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Droplet/Polymerization Test
Today Andrew and I tried out a new setup for the microfludic device. Here is an image of the new setup:
The setup allows us to use reagent reservoirs without much loss. This also keeps air bubbles from the lines, and helps with the problem of fouling that occurs when air gets into the device. The setup uses a syringe as the reservoir, and plugs the top with a rubber stopper. Air is introduced by a needle poked through the top.
Protocol 1
First we tested the drop seq device using APS/TEMED initiaors. We have only two regulators, so the aqueous lines received the same pressure and the oil line received a different pressure.