Daniel:Notebook/ComboLock/Devices

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Revision as of 17:51, 12 March 2016 by >Djacobse (→‎Drop Seq Device)
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Microfluidic Devices for Combo-Lock

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This page details the microfluidic devices used for the Combo-lock project. I have tried to include specifications whenever possible.

Drop Seq Device

Some specs for the Drop Seq device from the Macosko et al 2013 paper. Below is an image of a single device. A chip contains several dozen of these devices.

As can be seen, 4 lines are used, 3 inlets and an outlet. Two of the inlets are aqueous, the third is oil for droplet formation.

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The second thumbnail image shows the drop seq device junctions more closely. The first junction combines the two aqueous solutions; in the original paper this combines a cell solution with beads in a lysis buffer solution. See Combo-Lock protocols for details on which solutions I use. The second junction combines the mixed aqueous phases with the oil phase. This is where droplet formation occurs. Note that post-mixing there are grooves in the device. This facilitates mixing.