Daniel:Notebook/ComboLock/2016-3-15
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10% Acrylamide VA-044
From yesterday's results we decided to increase the acrylamide concentration in the gel solution in order to make up for a lack of formaldehyde/tissue.
VA-044/10% Acrylamide test
For test 2 we used the same setup as test 1, but with the following recipe for the VA-044 gel mix:
Reagent | Stock Concentration | Final Concentration | Amount added |
Acrylamide | 40% | 9% | 6.75 mL |
Acrylamide:Bis (19:1) | 40% (38:2) | 0.95:0.05 (%) | 0.75 mL |
VA-044 | ----- | 0.25% (m/v) | 0.075 g |
PBS | 10X | 1X | 3 mL |
ddH2O | ----- | ----- | 9.5 mL |
Total | 30 mL |
This is a slight modification from yesterday's recipe. The idea is to be able to incorporate gel loading dye along with the solution. This will hopefully allow better visualization of droplets until we get the dyes we ordered. Also, the volume has been scaled down to 30 mL, and prior to loading in the syringe, loading dye and solution should be mixed 1:2.
Run Parameters
- Line 1: Oil (on ice); 12 psi; ~2 mL
- Line 2: Aqueous (VA-044P; on ice); 2.5 psi; ~1 mL