Daniel:Notebook/ComboLock/2016-3-16
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Drop Seq Test (Started 03/15/2016)
After yesterday's results it would appear that we may have polyacrylamide droplets. However, I think the best test is a chemical one, so we'll be following the Macosko protocol for droplet breakage. This protocol uses Perfluoro-1-octanol to break apart droplets. If the droplets did form PA beads, then the octanol shouldn't have an effect. If they didn't the droplets should disappear.
Protocol
- Wash 3X with Mineral Oil (no Span)
- Remove oil phase with a pipette, and add in ~500 uL mineral oil
- Pipette the oil and beads gently to mix; repeat
- 1.1 and 1.2