CRISPR Library Design Pipeline
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The process for designing the 05/05/2015 Library Order and 02/17/2016 Library Order included:
- Create a manually curated gene list for each library.
- Pick 3-4 gRNAs per gene with the highest score from this File:GRNA spacer file.txt
- Add scaffold sequences and desired barcodes. The barcode list can be found in File:GRNA bc25mers.txt. The scaffold sequences can be found on this page: gRNA Scaffold Sequences.