Daniel:Protocols/LatchPadlock

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Revision as of 23:43, 8 June 2016 by >Djacobse (→‎Protocol)
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Latch and Padlock Hybridization

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Protocol

  1. Start from hybridized C Probes or antibodies
    1. Make sure probes have been washed as per the protocol
  2. After washing, incubate with 100 nM insert/backbone oligos for 30 min at 37C
  3. Wash twice


Buffers

  1. Insert/backbone Hybridization Buffer
    1. 100 nM insert/backbone oligos
    2. 1X SSC
    3. 40 U/mL RNasin
    4. PBS
  2. Wash Buffer
    1. PBS
    2. 0.1% Tween
    3. 4 U/mL RNasin