Daniel:Notebook/ComboLock/2016-8-17

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Positive Control Amplicon Production (Started yesterday)

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Protocol

  1. Size select gel
    1. Mix 30 uL TBE, 6 uL loading dye, and 4 uL sample for C1-C2 and C3-C2
    2. Mix 15 uL TBE, 3 uL loading dye, and 2 uL ladder
    3. Add 20 uL to each lane (2 lanes for C1-C2 and C3-C2)
    4. Run the gel at 250V for 25 min
    5. Stain with 3 uL SYBR gold for 3 min
    6. Image, and extract bands between 100bp and 125 bp; collect the gel bands in the same tube
    7. Shred the gel by centrifuging at 14000rpm for 1 min 30 sec
    8. Incubate in 500 uL TBE for 1 hour at 37C with vigorous shaking