Daniel:Notebook/ComboLock/2016-8-17
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Positive Control Amplicon Production (Started yesterday)
Protocol
- Size select gel
- Mix 30 uL TBE, 6 uL loading dye, and 4 uL sample for C1-C2 and C3-C2
- Mix 15 uL TBE, 3 uL loading dye, and 2 uL ladder
- Add 20 uL to each lane (2 lanes for C1-C2 and C3-C2)
- Run the gel at 250V for 25 min
- Stain with 3 uL SYBR gold for 3 min
- Image, and extract bands between 100bp and 125 bp; collect the gel bands in the same tube
- Shred the gel by centrifuging at 14000rpm for 1 min 30 sec
- Incubate in 500 uL TBE for 1 hour at 37C with vigorous shaking