Daniel:Notebook/ComboLock/2016-9-29

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Revision as of 17:20, 29 September 2016 by >Djacobse (→‎Results)
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Positive Control Amplicon Production (PCAmp4; Started yesterday)

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Protocol

  1. Size Selection
    1. Centrifuge at 12000 rpm for 1.5 minutes
    2. Extract the supernatant and place into NanoSep column
    3. Centrifuge at 12000 rpm for 1.5 minutes
  2. Ethanol Precipitation
    1. Move sample to a fresh 2 mL tube
    2. Add 1250 uL 100% EtOH, 50 uL 3M NaOAc and 2 uL glycoblue
    3. Store at for 6 hours
    4. Pellet by centrifuging for 25 minutes at 12000rpm at 4C
    5. Remove supernatant and add 750 uL chilled 70% EtOH
    6. Centrifuge for 12 minutes at 12000rpm at 4C
    7. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    8. Add 50 uL nfH2O and resuspend every tube in series;
    9. Measure final concentration in nanodrop

Results

Sample ng/uL ssDNA
C4-C2 Amplicon (Final)