Alice:LabNotes/2007-12-5
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The sequencing results finally worked!
Sample | SNP | Sequencing result | |
1 | rs3753565 | yes | |
2 | rs2295680 | yes | |
3 | rs6103 | no | |
4 | rs1264899 | yes | |
5 | rs1264899 | yes | |
6 | rs1264899 | yes | |
7 | rs1264899 | yes | |
Sample 1
File:SNP 1.png
Sample | peak location | base pair | base pair | peak value | |
ZL1-cust.ab1 | 92 | N | C | 255 | |
ZL1-cust.ab1 | 94 | C | C | 695 | |
ZL1-cust.ab1 | 92 | N | T | 214 | |
ZL1-cust.ab1 | 93 | T | T | 613 | |
peak ratio: 255/695 : 214/613 = 1.051 : 1
Sample 2
File:SNP 2.png
Sample | peak location | base pair | base pair | peak value | |
ZL2-cust.ab1 | 96 | A | G | 190 | |
ZL2-cust.ab1 | 94 | G | G | 724 | |
ZL2-cust.ab1 | 96 | A | A | 209 | |
ZL2-cust.ab1 | 95 | A | A | 647 | |
peak ratio: 190/724 : 209/647 = 0.811 : 1
Sample 4
File:SNP 4.png
Sample | peak location | base pair | base pair | peak value | |
ZL4-cust.ab1 | 88 | C | C | 763 | |
ZL4-cust.ab1 | 89 | C | C | 731 | |
ZL4-cust.ab1 | 88 | C | T | 58 | |
ZL4-cust.ab1 | 87 | T | T | 475 | |
peak ratio: 763/731 : 58/475 = 8.557 : 1
Sample 5
File:SNP 5.png
Sample | peak location | base pair | base pair | peak value | |
ZL5-cust.ab1 | 87 | T | T | 386 | |
ZL5-cust.ab1 | 86 | T | T | 342 | |
ZL5-cust.ab1 | 87 | T | C | 161 | |
ZL5-cust.ab1 | 88 | C | C | 583 | |
peak ratio: 386/342 : 161/583 = 4.091 : 1
Sample 6
File:SNP 6.png
Sample | peak location | base pair | base pair | peak value | |
ZL6-cust.ab1 | 150 | T | T | 746 | |
ZL6-cust.ab1 | 149 | T | T | 588 | |
ZL6-cust.ab1 | 150 | T | C | 199 | |
ZL6-cust.ab1 | 151 | C | C | 1021 | |
peak ratio: 746/588 : 199/1021 = 6.503 : 1
Sample 7
File:SNP 7.png
Sample | peak location | base pair | base pair | peak value | |
ZL7-cust.ab1 | 197 | A | G | 234 | |
ZL7-cust.ab1 | 196 | G | G | 476 | |
ZL7-cust.ab1 | 197 | A | A | 1358 | |
ZL7-cust.ab1 | 198 | A | A | 790 | |
peak ratio: 234/476 : 1358/790 = 0.286 : 1
The two samples we are really interested in are sample #6 and #7. The sample is GM20431 cDNA with rs 1264899 forward and reverse primer. The SNP location is rs 1264899. Then we will use software PeakPicker to compare and quantize the peaks.
Here is the files of the original sequencing result files: