Kun:LabNotes/ExonomeSeq/2007-12-17
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Exp.1 Start another round of E55kv1 probe production
PCR (with NEB Taq master mix)
x1 x32 E55k1(20nM) 0.1ul 3.2ul 2x Taq master mix 50ul 1600ul 100uM pAP1V6U 0.3ul 9.6ul 100uM phosAP2V6 0.3ul 9.6ul H2O 49.3ul 1600ul
94C 3min -> 18 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold