Kun:LabNotes/ExonomeSeq/2007-12-23

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Exp.1 PCR on the capturing reaction started on 12/21

    Template:              10ul
    10X JumpStart buffer:   9ul
    25mM dNTP               1ul
    100uM AmpFV6.2Sol     0.4ul
    100uM AmpRV6.2Sol     0.4ul
    50X SYBG I            0.4ul
    JumpStart Taq           1ul
    H2O                    79ul

94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.

  File:2007-12-24-E55kv1-exon-capture-PCR.png

PAGE purification.

  File:2007-12-27-E55kv1-PCR-PAGE.png