Kun:LabNotes/ExonomeSeq/2007-12-31

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Revision as of 01:26, 1 January 2008 by KunZhang (talk | contribs) (New page: ==Colony PCR== Perform PCR on 16 colonies from each of the three plates: E55kv1(200+350), E55kv1(250+450), CpGPCR. 2X Taq master mix: 15ul 10uM M13 primer mix: 1ul H2O...)
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Colony PCR

Perform PCR on 16 colonies from each of the three plates: E55kv1(200+350), E55kv1(250+450), CpGPCR.

    2X Taq master mix:    15ul
    10uM M13 primer mix:   1ul
    H2O:                  14ul

94C 3min -> (94C 30sec -> 54C 30sec -> 72C 45sec) x 35 -> 72C 3min -> 4C hold.