AlanFung:Protocol/Genome Analyzer

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Preparing Reagents for the Genome Analyzer

  • Genome analyzer Reagent Compartment & Reagent Position

File:Reagent Position.jpg

  • Position of each reagent in the Genome Analyzer
NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
Position    Reagent              Volume              Container
1           Incorporation Mix    Varies w/ cycle #   50ml amber tube
2           DI H2O                                   259ml bottle
3           Scanning Mix         50ml                50ml tube
4           High Salt Buffer                         250ml bottle
5           Incorporation buffer 150ml               250ml bottle
6           Cleavage Mix         50ml                50ml tube
7           Cleavage Buffer      70ml                250ml bottle